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Distribution of the actin cytoskeleton during the cell cycle of Yarrowia lipolytica and the visualization of the tubulin cytoskeleton by immunofluorescence
Author(s) -
Gausmann Ulrike,
Franzl Erika,
Kurischko Cornelia
Publication year - 1999
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199908)15:11<1079::aid-yea435>3.0.co;2-d
Subject(s) - biology , cytoskeleton , actin , microbiology and biotechnology , actin cytoskeleton , immunostaining , saccharomyces cerevisiae , yeast , yarrowia , tubulin , hypha , cell cycle , microtubule , cell , biochemistry , immunology , immunohistochemistry
Actin distribution was examined during the cell cycle of the dimorphic yeast Yarrowia lipolytica , showing the correlation between bud growth, nuclear migration and rearrangement of the actin cytoskeleton. The results correspond with observations made in cells of Saccharomyces cerevisiae , S. uvarum and Candida albicans . Localization of actin was also determined in hyphal cells, where actin is stained predominantly in the tip and also at the septum of hyphae. The standard methods used for tubulin immunostaining in S. cerevisiae and C. albicans cells were adapted for application in Y. lipolytica . Copyright © 1999 John Wiley & Sons, Ltd.