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Construction and genetic analysis of S. cerevisiae deletants of six novel ORFs from chromosome II
Author(s) -
Malagón Francisco,
Aguilera Andrés
Publication year - 1999
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199907)15:10b<955::aid-yea395>3.0.co;2-2
Subject(s) - orfs , biology , plasmid , genetics , gene , chromosome , saccharomyces cerevisiae , open reading frame , peptide sequence
We have constructed S. cerevisiae strains carrying genomic deletions of six ORFs from the left arm of chromosome II ( YBL018c , YBL019w , YBL024w , YBL042c , YBL043w and YBL046w ) in both FY1679 and W303 backgrounds. We have found that YBL018c is an essential gene in yeast, whereas the other five genes are non‐essential. We have developed plasmids carrying deletion cassettes that can be used to delete any of the six genes in S. cerevisiae by transforming to G418‐resistance, as well as centromeric plasmids containing the cognate genes. Copyright © 1999 John Wiley & Sons, Ltd.