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Cloning of a centromere binding factor 3d ( CBF3D ) gene from Candida glabrata
Author(s) -
Stoyan Tanja,
Eck Raimund,
Lechner Johannes,
Hemmerich Peter,
Künkel Waldemar,
Diekmann Stephan
Publication year - 1999
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19990630)15:9<793::aid-yea415>3.0.co;2-g
Subject(s) - biology , genetics , gene , genbank , open reading frame , saccharomyces cerevisiae , candida glabrata , centromere , southern blot , microbiology and biotechnology , molecular cloning , genomic dna , cloning (programming) , accession number (library science) , exon , sequence analysis , genomic library , mutant , peptide sequence , chromosome , candida albicans , computer science , programming language
The gene encoding centromere binding factor 3d ( CBF3D ) of the human pathogenic yeast Candida glabrata has been isolated by hybridization of Saccharomyces cerevisiae CBF3D ( ScCBF3D ) DNA to a C. glabrata partial genomic library. Sequence analysis revealed a 540 bp open reading frame encoding a protein of 179 amino acids with a calculated molecular mass of 20·9 kDa. The amino acid sequence is highly homologous (78·6% identity) to ScCbf3d and 48·3% identical to the human homologue p19 ( SKP1 ). Southern blot analysis indicates that CgCbf3d is encoded by an unique nuclear gene. The cloned CgCBF3D gene can functionally substitute the S. cerevisiae homologue in a S. cerevisiae CBF3D ‐deletion mutant. The GenBank Accession No. for this gene is AF 072472. Copyright © 1999 John Wiley & Sons, Ltd.