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Characterization of the histidine mutants of Kluyveromyces lactis
Author(s) -
Zonneveld B. J. M.,
Tizzani L.,
WésolowskiLouvel M.
Publication year - 1999
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19990330)15:5<377::aid-yea381>3.0.co;2-4
Subject(s) - complementation , kluyveromyces lactis , biology , histidine , genetics , gene , saccharomyces cerevisiae , mutant , protein fragment complementation assay , heterologous , homology (biology) , mutation , amino acid
Thirty‐eight different histidine mutations of Kluyveromyces lactis were isolated and genetically characterized. All of the mutations were nuclear recessive alleles. They turned out to belong to seven different complementation groups, designated hisA1 to hisA7 . Five of these genes have been cloned by in vivo complementation of the Klhis mutations. Their homology to some of the histidine genes of Saccharomyces cerevisiae was confirmed by heterologous complementation. However, one of these KlHIS genes did not complement any mutation in the seven known histidine biosynthetic enzymes encoding genes ( his1–his7 ) of S. cerevisiae . Copyright © 1999 John Wiley & Sons, Ltd.