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Asymmetric distribution of phosphatidylethanolamine in C. albicans : possible mediation by CDR1 , a multidrug transporter belonging to ATP binding cassette (ABC) superfamily
Author(s) -
Dogra Smriti,
Krishnamurthy Shankarling,
Gupta Vinita,
Dixit Bharat L.,
Gupta C. M.,
Sanglard Dominique,
Pprasad Rajendra
Publication year - 1999
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19990130)15:2<111::aid-yea350>3.0.co;2-e
Subject(s) - biology , atp binding cassette transporter , candida albicans , transporter , yeast , corpus albicans , biochemistry , microbiology and biotechnology , gene
By using two molecular probes, we demonstrate that only 4% of total phosphatidylethanolamine (PtdEtn) in the plasma membrane (PM) of a human pathogenic yeast, Candida albicans , is present in its external half. Evidence is presented to show that the availability of PtdEtn could be related to the expression of a multidrug transporter CDR1 of C. albicans , and the process is energy‐dependent. A homozygous CDR1 disruptant strain of C. albicans shows almost 23% reduction in the external labelling of PtdEtn. This report shows that, similar to human MDRs, yeast multidrug transporter could also be involved in aminophospholipid translocation. Copyright © 1999 John Wiley & Sons, Ltd.