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Isolation and sequence analysis of the orotidine‐5'‐phosphate decarboxylase gene ( URA3 ) of Candida utilis . Comparison with the OMP decarboxylase gene family
Author(s) -
Rodríguez Luis,
Chávez Francisco P.,
González Maria E.,
Basabe Liliana,
Rivero Tanilo
Publication year - 1998
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199811)14:15<1399::aid-yea324>3.0.co;2-d
Subject(s) - ura3 , biology , gene , sequence analysis , complementation , genetics , microbiology and biotechnology , biochemistry , peptide sequence , saccharomyces cerevisiae , mutant
Abstract The URA3 gene of Candida utilis encoding orotidine‐5′‐phosphate decarboxylase enzyme was isolated by complementation in Escherichia coli pyrF mutation. The deduced amino‐acid sequence is highly similar to that of the Ura3 proteins from other yeast and fungal species. An extensive analysis of the family of orotidine‐5′‐phosphate decarboxylase is shown. The URA3 gene of C. utilis was able to complement functionally the ura3 mutation of Saccharomyces cerevisiae. The sequence presented here has been deposited in the EMBL data library under Accession Number Y12660. © 1998 John Wiley & Sons, Ltd.