Premium
Molecular cloning and sequence analysis of the Schizosaccharomyces pombe ade10 + gene
Author(s) -
Liedtke Christian,
Schmidt Henning
Publication year - 1998
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(1998100)14:14<1307::aid-yea308>3.0.co;2-4
Subject(s) - schizosaccharomyces pombe , biology , open reading frame , nucleic acid sequence , gene , genetics , accession number (library science) , peptide sequence , cloning (programming) , molecular cloning , schizosaccharomyces , sequence analysis , gene product , microbiology and biotechnology , saccharomyces cerevisiae , genbank , gene expression , computer science , programming language
We have cloned and sequenced the Schizosaccharomyces pombe ade10 gene encoding 5‐phosphoribosyl‐4‐carboxamide 5‐aminoimidazole transformylase inosine monophosphate cyclohydrolase. The sequence has an uninterrupted open reading frame of 1755 nucleotides corresponding to 585 amino acid residues. The deduced amino acid sequence shows a high degree of similarity to the purH gene product of many species, including Saccharomyces cerevisiae , human, chicken and Escherichia coli. Moreover our data indicate that intrachromosomal recombination in Schiz. pombe is enhanced if the ade10 gene product is defective. The sequence has been submitted to the EMBL data library under Accession Number Y16419. © 1998 John Wiley & Sons, Ltd.