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Cloning and characterization of the Hansenula polymorpha homologue of the Saccharomyces cerevisiae PMR1 gene
Author(s) -
Kang Hyun Ah,
Kim JeongYoon,
Ko SuMin,
Park Cheon Seok,
Ryu Dewey D. Y.,
Sohn JungHoon,
Choi EuiSung,
Rhee SangKi
Publication year - 1998
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19980930)14:13<1233::aid-yea322>3.0.co;2-y
Subject(s) - biology , saccharomyces cerevisiae , gene , gene product , mutant , yeast , microbiology and biotechnology , molecular cloning , dna , genetics , peptide sequence , gene expression
A gene homologous to Saccharomyces cerevisiae PMR1 has been cloned in the methylotrophic yeast Hansenula polymorpha . The partial DNA fragment of the H. polymorpha homologue was initially obtained by a polymerase chain reaction and used to isolate the entire gene which encodes a protein of 918 amino acids. The putative gene product contains all ten of the conserved regions observed in P‐type ATPases. The cloned gene product exhibits 60·3% amino acid identity to the S. cerevisiae PMR1 gene product and complemented the growth defect of a S. cerevisiae pmr1 null mutant in the EGTA‐containing medium. The results demonstrate that the H. polymorpha gene encodes the functional homologue of the S. cerevisiae PMR1 gene product, a P‐type Ca 2+ ‐ATPase. The DNA sequence of the H. polymorpha homologue has been submitted to GenBank with the Accession Number U92083. © 1998 John Wiley & Sons, Ltd.