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Cloning and sequence analysis of the gene encoding invertase ( INV 1) from the yeast Candida utilis
Author(s) -
Chávez Francisco P.,
Pons Tirso,
Delgado Julio M.,
Rodríguez Luis
Publication year - 1998
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19980930)14:13<1223::aid-yea301>3.0.co;2-3
Subject(s) - biology , gene , invertase , genetics , coding region , nucleic acid sequence , peptide sequence , signal peptide , yeast , saccharomyces cerevisiae , amino acid , microbiology and biotechnology , homology (biology) , molecular cloning , biochemistry , enzyme
The gene INV1 encoding invertase from the yeast Candida utilis has been cloned using a homologous PCR hybridization probe, amplified with two sets of degenerate primers designed considering sequence comparisons between yeast invertases. The cloned gene was sequenced and found to encode a polypeptide of 533 amino acids that contain a 26 amino‐acid signal peptide and 12 potential N‐glycosylation sites. The nucleotide sequences of the 5′ and 3′ non‐coding regions were found to contain motifs probably involved in initiation, regulation and termination of gene transcription. The amino‐acid sequence shows significant identity with other yeast, bacterial and plant β‐fructofuranosidases. The INV1 gene from C. utilis was able to complement functionally the suc2 mutation of S. cerevisiae . The sequence presented here has been deposited in the EMBL data library under Accession Number Y12659. © 1998 John Wiley & Sons, Ltd.