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Multiple regulatory proteins mediate repression and activation by interaction with the yeast Mig1 binding site
Author(s) -
Wu Jianping,
Trumbly Robert J.
Publication year - 1998
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199808)14:11<985::aid-yea294>3.0.co;2-c
Subject(s) - biology , psychological repression , yeast , binding site , saccharomyces cerevisiae , genetics , dna binding protein , microbiology and biotechnology , gene , transcription factor , gene expression
A major mediator of glucose repression in yeast is Mig1, a zinc finger protein that binds to a GC‐rich recognition sequence found upstream of many glucose‐repressible genes. Because these Mig1 sites are found upstream of genes under different modes of regulation, we studied regulation of transcription mediated by an isolated Mig1 site placed upstream of a reporter gene under control of UAS CYC1 . The Mig1 site responded appropriately to glucose control and regulatory mutations, including snf1 , reg1 , cyc8 , and tup1 , mimicking the behavior of the SUC2 gene. Deletion of the MIG1 ‐coding gene reduced but did not eliminate glucose repression mediated by the Mig1 site. Complete loss of repression was seen in a mig1 mig2 double mutant. When the UAS CYC1 was replaced by UAS ADH1 in the reporter plasmid, the Mig1 site activated transcription under most conditions. Mutations of the two Mig1 binding sites in the SUC2 promoter resulted in loss of activation of SUC2 expression. These results suggest the presence of an unknown activator or activators that binds to the Mig1 site. The activator is not any of the proteins previously proposed to bind to this site, including Mig1, Mig2, Msn2, or Msn4. Band shift assays showed that Mig1 is the major protein in yeast cell extracts that binds to the Mig1 site in vitro . This binding is not regulated by glucose or mutations in CYC8 or TUP1 . © 1998 John Wiley & Sons, Ltd.