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Studies of astaxanthin biosynthesis in Xanthophyllomyces dendrorhous (Phaffia rhodozyma ). Effect of inhibitors and low temperature
Author(s) -
Ducrey Sanpietro Luis M.,
Kula M.R.
Publication year - 1998
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199808)14:11<1007::aid-yea307>3.0.co;2-u
Subject(s) - diphenylamine , phytoene desaturase , astaxanthin , biosynthesis , lycopene , biology , carotenoid , biochemistry , phytoene , nicotine , enzyme , chemistry , organic chemistry , neuroscience
The effect of nicotine and diphenylamine on astaxanthin biosynthesis in Xanthophyllomyces dendrorhous was studied. The effects were analysed under standard and low temperature conditions. It was found that 10 mm‐nicotine inhibits the cyclization of lycopene and de novo protein synthesis was not needed to reverse the inhibition. The oxidation of β‐carotene was irreversibly inhibited by 10 μ M ‐diphenylamine while the dehydrogenation of phytoene was reversibly inhibited by 60 μ M ‐diphenylamine. The simultaneous exposure to low temperature (4°C) overcomes the inhibition of β‐carotene oxidation at low diphenylamine concentration. © 1998 John Wiley & Sons, Ltd.