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Isolation and nucleotide sequence of the gene encoding phosphoenolpyruvate carboxykinase from Kluyveromyces lactis
Author(s) -
Kitamoto Hiroko K.,
Ohmomo Sadahiro,
Iimura Yuzuru
Publication year - 1998
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199807)14:10<963::aid-yea282>3.0.co;2-x
Subject(s) - biology , kluyveromyces lactis , phosphoenolpyruvate carboxykinase , nucleic acid sequence , gene , open reading frame , amplicon , saccharomyces cerevisiae , genetics , homology (biology) , peptide sequence , microbiology and biotechnology , biochemistry , polymerase chain reaction
The KlPCK1 gene encoding phosphoenolpyruvate carboxykinase (PEPCK; ATP‐dependent) was cloned from the Kluyveromyces lactis genome using a PCR amplicon from Saccharomyces cerevisiae PCK1 gene as a probe. A DNA fragment of about 4·8 kb containing KlPCK1 complemented PEPCK activity of the mutant of S. cerevisiae defective in PEPCK. The KlPCK1 gene has an open reading frame of 1629 bp (543 amino acids). The KlPCK1 nucleotide sequence and deduced amino acid sequence showed 76% and 84% homologies to those of S. cerevisiae PCK1 , respectively. Multiple alignment of ATP‐dependent PEPCK genes shows highly conserved regions. The nucleotide sequence of KlPCK1 has been submitted to the DDBJ/GenBank/EMBL data bank with Accession Number U88575. © 1998 John Wiley & Sons, Ltd.