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Identification and characterization of the KlCMD1 gene encoding Kluyveromyces lactis calmodulin
Author(s) -
Rayner Timothy F.,
Stark Michael J. R.
Publication year - 1998
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19980630)14:9<869::aid-yea278>3.0.co;2-u
Subject(s) - biology , calmodulin , identification (biology) , gene , computational biology , genetics , microbiology and biotechnology , biochemistry , botany , enzyme
The KlCMD1 gene was isolated from a Kluyveromyces lactis genomic library as a suppressor of the Saccharomyces cerevisiae temperature‐sensitive mutant spc110‐124 , an allele previously shown to be suppressed by elevated copy number of the S. cerevisiae calmodulin gene CMD1 . The KlCMD1 gene encodes a polypeptide which is 95% identical to S. cerevisiae calmodulin and 55% identical to calmodulin from Schizosaccharomyces pombe . Complementation of a S. cerevisiae cmd1 deletion mutant by KlCMD1 demonstrates that this gene encodes a functional calmodulin homologue. Multiple sequence alignment of calmodulins from yeast and multicellular eukaryotes shows that the K. lactis and S. cerevisiae calmodulins are considerably more closely related to each other than to other calmodulins, most of which have four functional Ca 2+ ‐binding EF hand domains. Thus like its S. cerevisiae counterpart Cmd1p, the KlCMD1 product is predicted to form only three Ca 2+ ‐binding motifs. The KlCMD1 sequence has been assigned Accession Number AJ002021 in the EMBL/GenBank database. © 1998 John Wiley & Sons, Ltd.