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Development of a transformation system for the multinuclear yeast Dipodascus (Endomyces) magnusii
Author(s) -
Adamíková L'ubica,
Griač Peter,
Tomáška L'ubomír,
Nosek Jozef
Publication year - 1998
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19980630)14:9<805::aid-yea279>3.0.co;2-8
Subject(s) - biology , plasmid , electroporation , selectable marker , autonomously replicating sequence , transformation (genetics) , genetics , dna , origin of replication , shuttle vector , vector (molecular biology) , dna replication , microbiology and biotechnology , yeast , computational biology , recombinant dna , gene
We have developed the first system for genetic transformation of the multinuclear yeast Dipodascus magnusii . The system is based on a dominant selectable marker and an autonomously replicating sequence. We have constructed a plasmid vector which contains a marker conferring resistance to zeocin and the segment of non‐transcribed spacer of D. magnusii ribosomal DNA which supports the autonomous replication of plasmid DNA in yeast cells. Plasmid DNA has been transferred into D. magnusii cells by electroporation. The DNA sequence which is described in this article has been deposited in the EMBL data library under Accession Number Y14587. © 1998 John Wiley & Sons, Ltd.