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Isolation and characterization of the Candida albicans SEC4 Gene
Author(s) -
Clément Martin,
Fournier Hélène,
De Repentigny Louis,
Belhumeur Pierre
Publication year - 1998
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199805)14:7<675::aid-yea252>3.0.co;2-9
Subject(s) - biology , candida albicans , saccharomyces cerevisiae , gene , gene product , mutant , corpus albicans , yeast , biochemistry , microbiology and biotechnology , genetics , gene expression
The SEC4 gene product is a major component of the protein secretion machinery. More specifically, it is believed to play a pivotal role in targeting and fusion of secretory vesicles to the plasma membrane. Its recently described implication with the Saccharomyces cerevisiae Rho3p, which is required for directing growing points during bud formation, has prompted us to investigate the role and function of Sec4p in the morphological changes of the yeast pathogen Candida albicans . We have therefore cloned the C. albicans SEC4 gene. It encodes a 210 amino acids long protein sharing up to 75% homology to the S. cerevisiae homolog, when conserved changes are allowed. Its RNA is constitutively expressed in C. albicans grown under various physiological conditions. We also show that it can functionally complement a S. cerevisiae sec4 thermosensitive mutant. The sequence of the C. albicans SEC4 gene has been deposited in GenBank under Accession Number AF017183. © 1998 John Wiley & Sons, Ltd.