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Identification of a putative histidine kinase two‐component phosphorelay gene ( CaHK1 ) in Candida albicans
Author(s) -
Calera Jose A.,
Choi Gil H.,
Calderone Richard A.
Publication year - 1998
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199805)14:7<665::aid-yea246>3.0.co;2-#
Subject(s) - histidine kinase , biology , response regulator , histidine , gene , homology (biology) , candida albicans , two component regulatory system , biochemistry , sh3 domain , kinase , microbiology and biotechnology , genetics , amino acid , proto oncogene tyrosine protein kinase src , mutant
We have cloned and analysed the sequence of a putative histidine kinase, two‐component gene ( CaHK1 ) from Candida albicans . This gene encodes a 2471 amino acid protein (Cahk1p) with an estimated molecular mass of 281·8 kDa. A homology search of Cahk1p with other proteins in the databases showed that Cahk1p exhibits the greatest homology at its C‐terminus with both the sensor and regulator components of prokaryotic and eukaryotic two‐component histidine kinases. A further analysis of this homology showed that the Cahk1p possessed both sensor and regulator domains in the same polypeptide. Also, Cahk1p is likely to be a soluble protein. The sensor kinase domain of Cahk1p contains conserved motifs that are characteristic of all histidine kinase proteins, including the putative histidine which is believed to be autophosphorylated during activation, ATP binding motifs and others (F‐ and N‐motifs), with unknown function. The Cahk1p regulator domain also contains conserved aspartate and lysine residues and the putative aspartate, which is secondarily phosphorylated by the autophosphorylated histidine. Finally, according to the codon usage frequency of the CaHK1 gene in comparison with other genes from C. albicans , there would appear to be a low level of expression of the gene. The accession number for the described sequence is AF013273, as filed in the EMBL/GenBank/DDBJ database. © 1998 John Wiley & Sons, Ltd.