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Identification and analysis of homologues of Saccharomyces cerevisiae Spt3 suggest conserved functional domains
Author(s) -
Madison Jon M.,
Winston Fred
Publication year - 1998
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19980330)14:5<409::aid-yea237>3.0.co;2-x
Subject(s) - kluyveromyces lactis , biology , schizosaccharomyces pombe , saccharomyces cerevisiae , genetics , kluyveromyces , conserved sequence , yeast , gene , peptide sequence
Spt3 of Saccharomyces cerevisiae is a factor required for normal transcription from particular RNA polymerase II‐dependent promoters. As a step towards analysing Spt3 structure–function relationships, we have identified and studied Spt3 homologues from three other yeasts: Kluyveromyces lactis, Clavispora opuntiae and Schizosaccharomyces pombe . Alignment of their predicted amino acid sequences shows an overall identity of 30% between all four homologues and suggests that three conserved domains are present in Spt3. When tested for function in S. cerevisiae , K. lactis SPT3 was shown to fully complement and S. pombe SPT3 to partially complement an spt3 Δ mutation. These data demonstrate that Spt3 is functionally conserved among distantly related yeasts. The new sequences have been entered in GenBank: AF005930 ( K. lactis SPT3 ), AF005932 ( C. opuntiae SPT3 ) and AF005931 ( S. pombe SPT3 ). © 1998 John Wiley & Sons, Ltd.