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Isolation and characteristics of yeasts able to grow at low concentrations of nutrients
Author(s) -
Kimura Yoshio,
Nakano Yoshinaga,
Fujita Kiyoto,
Miyabe Shinji,
Imasaka Shigeru,
Ishikawa Yasuko,
Sato Masayuki
Publication year - 1998
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199802)14:3<233::aid-yea216>3.0.co;2-1
Subject(s) - biology , yeast , dilution , nutrient , starvation , yeast extract , phosphate , inoculation , leucine , carbohydrate , food science , amino acid , microbiology and biotechnology , botany , zoology , biochemistry , ecology , fermentation , horticulture , physics , endocrinology , thermodynamics
Seven oligotrophic yeasts, which can grow in a 10 4 ‐fold dilution of malt–yeast–glucose–peptone medium (10 −4 YM), were mainly isolated from soil. These yeasts belong to the Cryptococcaceae. When inoculated at about 10 2 cells/ml in 10 −4 YM, the isolates grew to 1·4×10 3 –2·4×10 5 cells/ml after 3 days. Some culture collection yeasts fell into three groups according to their growth characteristics in 10 −4 YM, one group showing characteristics of the oligotrophic yeasts. The half‐saturation values of uptake by the five isolated oligotrophic yeasts for D‐glucose, L‐leucine and L‐amino acids were 6·0–25·0, 1·7–43·3 and 3·5–21·6 μM, respectively. The oligotrophic yeasts suspended in 10 mM‐phosphate buffer (pH 6·0) had high tolerances for starvation, and remained more than 15% viable after 90 days of starvation. © 1998 John Wiley & Sons, Ltd.