Premium
Molecular cloning and sequencing of a chitin synthase gene (CHS2) of Paracoccidioides brasiliensis
Author(s) -
NiñoVega Gustavo A.,
Buurman Ed T.,
Gooday Graham W.,
SanBlas Gioconda,
Gow Neil A. R.
Publication year - 1998
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19980130)14:2<181::aid-yea202>3.0.co;2-d
Subject(s) - biology , chitin synthase , gene , molecular cloning , cloning (programming) , genetics , chitin , computational biology , biochemistry , gene expression , chitosan , computer science , programming language
The nucleotide sequence of a chitin synthase gene ( CHS2 ) of the dimorphic fungal human pathogen Paracoccidioides brasiliensis has been determined. The deduced amino acid sequence of Chs2p consists of 1043 residues and is highly homologous to other class II fungal chitin synthases. Computational structural analyses suggest very high similarity to other fungal chitin synthases with a highly variable region at the cytosolic amino‐terminal region which may be related to its possible zymogenic nature, and the putative catalytic region close to seven membrane‐spanning regions at the carboxyl terminus. The nucleotide sequence of CHS2 and its flanking regions has been submitted to GenBank under Accession Number Y09231. © 1998 John Wiley & Sons, Ltd.