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Mutations in five loci affecting GAP1 ‐independent uptake of neutral amino acids in yeast
Author(s) -
Jørgensen Marianne U.,
Bruun Marianne B.,
Didion Thomas,
KiellandBrandt Morten C.
Publication year - 1998
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19980130)14:2<103::aid-yea203>3.0.co;2-c
Subject(s) - biology , amino acid , valine , complementation , isoleucine , leucine , mutant , biochemistry , genetics , gene , saccharomyces cerevisiae , amino acid transporter , branched chain amino acid , amino acid synthesis , methionine , microbiology and biotechnology , lysine , transporter
Abstract In order to identify genes involved in uptake of isoleucine, leucine and valine in Saccharomyces cerevisiae we isolated mutants that, on a complex medium, were sensitive to an inhibitor of the biosynthesis of the branched‐chain amino acids. Mutants that in a secondary screen showed reduced uptake of isoleucine, leucine and valine when growing in synthetic complete medium were further characterized. Genetic analysis identified five loci, named ssy1 through ssy5. ssy2 corresponds to the previously characterized bap1 mutation, which we recently have found to be allelic to stp1. ssy1 , ssy3 and ssy5 exhibit a reduced uptake of phenylalanine, methionine and threonine, as well. Furthermore, they are resistant to several neutral amino acid analogs. ssy4 only affects uptake of few neutral amino acids and is as sensitive as the wild type to the amino acid analogs tested. It was previously found that a C‐terminal truncation of 29 codons of BAP2 , which encodes a b ranched‐chain a mino acid p ermease, results in increased uptake of the branched‐chain amino acids. We find epistasis of the C‐terminally truncated BAP2 gene over the ssy4 mutation, while the other ssy mutations are epistatic over the truncated BAP2 gene. SSY1 , SSY3 and SSY5 were cloned from a low‐copy genomic library by complementation of the mutants. The SSY3 gene and the SSY5 gene show no significant homology to any sequence in the databases. SSY1 is a member of the major family of genes encoding amino acid permeases in yeast. We discuss possible roles of Ssy1p in amino acid uptake. © 1998 John Wiley & Sons, Ltd.