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The Sge1 protein of Saccharomyces cerevisiae is a membrane‐associated multidrug transporter
Author(s) -
EhrenhoferMurray Ann E.,
Keller Seitz Monika U.,
Sengstag Christian
Publication year - 1998
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19980115)14:1<49::aid-yea199>3.0.co;2-t
Subject(s) - biology , major facilitator superfamily , saccharomyces cerevisiae , permease , yeast , lactose permease , biochemistry , ethidium bromide , multiple drug resistance , membrane protein , cell fractionation , membrane transport protein , transport protein , transporter , membrane , gene , dna , antibiotics
In this study, we report the further characterization of the Saccharomyces cerevisiae crystal violet‐resistance protein Sge1. Sge1 is a highly hydrophobic 59 kDa protein with 14 predicted membrane‐spanning domains. It shares homologies with several drug‐resistance proteins and sugar transporters of the major facilitator superfamily. Here, we have demonstrated that Sge1 is not only a crystal violet‐resistance protein, but that it also confers resistance to ethidium bromide and methylmethane sulfonate. Disruption of SGE1 leads to increased sensitivity towards all three compounds, thus designating Sge1 as a multiple drug‐resistance protein. Subcellular fractionation as well as immunolocalization on whole yeast cells demonstrated that Sge1 was tightly associated with the yeast plasma membrane. Furthermore, Sge1 was highly enriched in preparations of yeast plasma membranes. In analogy to other multidrug‐resistance proteins, we suggest that Sge1 functions as a drug export permease. © 1998 John Wiley & Sons, Ltd.