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Sequences of Saccharomyces cerevisiae 2 μm DNA improving plasmid partitioning in Hansenula polymorpha
Author(s) -
Bogdanova Aliona I.,
Kustikova Olga S.,
Agaphonov Michael O.,
TerAvanesyan Michael D.
Publication year - 1998
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19980115)14:1<1::aid-yea195>3.0.co;2-d
Subject(s) - biology , plasmid , dna , genetics , recombinant dna , saccharomyces cerevisiae , computational biology , gene
Insertion of the Hin dIII‐ Pst I fragment of Saccharomyces cerevisiae 2 μm DNA into the Hansenula polymorpha replicative plasmids decreases plasmid copy number and ensures their distribution to daughter cells at both mitotic and meiotic cell divisions. This suggests that the stabilization effect is caused by the improvement of plasmid partitioning. Deletion analysis revealed that the region of 2 μm DNA sequence responsible for the increase of mitotic stability of H. polymorpha plasmids involves the 2 μm STB locus and adjoining region. Further analysis demonstrated that the stabilization effect may depend on the number of 24–28 bp imperfect repeats which were found in several copies in the STB locus and adjoining region. © 1998 John Wiley & Sons, Ltd.