Premium
Saccharomyces carlsbergensis contains two functional genes encoding the Acyl‐CoA binding protein, one similar to the ACB1 gene from S. cerevisiae and one identical to the ACB1 gene from S. monacensis
Author(s) -
Børsting Claus,
Hummel Rene,
Schultz Emily R.,
Rose Timothy M.,
Pedersen Mogens B.,
Knudsen Jens,
Kristiansen Karsten
Publication year - 1997
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199712)13:15<1409::aid-yea188>3.0.co;2-a
Subject(s) - biology , gene , genetics , saccharomyces cerevisiae , saccharomyces , polyadenylation , gene expression
Saccharomyces carlsbergensis is an amphiploid, and it has previously been suggested that the genomes of S. carlsbergensis originate from S. cerevisiae and S. monacensis . We have cloned the ACB1 genes encoding the acyl‐CoA binding protein (ACBP) from S. carlsbergensis , S. cerevisiae and S. monacensis . Two genes were found in S. carlsbergensis and named ACB1 type 1 and type 2, respectively. The type 1 gene is identical to the S. cerevisiae ACB1 gene except for three substitutions, one single base pair deletion and one double base pair insertion, all located in the promoter region. The type 2 gene is completely identical to the S. monacensis ACB1 gene. These findings substantiate the notion that S. carlsbergensis is a hybrid between S. cerevisiae and S. monacensis .Both ACB1 type 1 and type 2 are actively transcribed in S. carlsbergensis and transcription is initiated at sites identical to those used for transcriptional initiation of the ACB1 genes in S. cerevisiae and S. monacensis , respectively. Two polyadenylation sites, spaced 225 bp apart, are present in the S. cerevisiae ACB1 gene. The upstream polyadenylation site is used exclusively during exponential growth, whereas both sites are utilized during later stages of growth. All sequence information is listed under EMBL Accession Numbers Y08687, Y08688, Y08689 and Y08690. © 1997 John Wiley & Sons, Ltd.