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Isolation of a putative prolyl‐tRNA synthetase ( CaPRS ) gene from Candida albicans
Author(s) -
Sentandreu Maria,
Elorza M. Victoria,
Sentandreu Rafael
Publication year - 1997
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199711)13:14<1375::aid-yea179>3.0.co;2-i
Subject(s) - biology , orfs , transfer rna , candida albicans , open reading frame , gene , genetics , amino acyl trna synthetases , accession number (library science) , amino acid , saccharomyces cerevisiae , biochemistry , dna , nucleic acid sequence , escherichia coli , peptide sequence , microbiology and biotechnology , rna , genbank
We have isolated a 4·0‐kb fragment from a genomic library of Candida albicans which contained two open reading frames (ORFs). One of them is homologous to a prolyl‐tRNA synthetase that catalyses the charging of a specific tRNA by proline ( CaPRS ). A deduced sequence of 575 amino acids representing a polypeptide of 66·2 kDa was determined. A FASTA search indicated that the CaPRSp had an overall similarity of 54·4% with the product of a Saccharomyces cerevisiae ORF (YER087) and 43·8% with the prolyl‐tRNA synthetase of Escherichia coli (COLIPRO). Consensus Class II aminoacyl‐tRNA synthetase sequences were identified by the PROSITE program. CaPRS was localized to chromosome R of the C. albicans genome and CaPRS DNA hybridized to a major RNA transcript of 1·7 kb under all conditions tested. The CaPRS sequence submitted to the EMBL data library is available under Accession Number U86341.© 1997 John Wiley & Sons, Ltd.