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The ALD6 gene of Saccharomyces cerevisiae encodes a cytosolic, Mg 2+ ‐activated acetaldehyde dehydrogenase
Author(s) -
Meaden Philip G.,
Dickinson Francis M.,
Mifsud Amparo,
Tessier Wayne,
Westwater John,
Bussey Howard,
Midgley Melvin
Publication year - 1997
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199711)13:14<1319::aid-yea183>3.0.co;2-t
Subject(s) - biology , aldehyde dehydrogenase , acetaldehyde , saccharomyces cerevisiae , biochemistry , mutant , amino acid , open reading frame , cytosol , dehydrogenase , alcohol dehydrogenase , homology (biology) , gene , microbiology and biotechnology , glutamate dehydrogenase , enzyme , peptide sequence , ethanol , glutamate receptor , receptor
The deduced translation product of an open reading frame on the left arm of chromosome XVI of Saccharomyces cerevisiae , with the systematic name of YPL061w, is 500 amino acids in length and shares significant homology with aldehyde dehydrogenases. Amino acids 2 to 16 of the protein encoded by YPL061w were found to be identical to the N‐terminal 15 amino acids of the purified cytosolic, Mg 2+ ‐activated acetaldehyde dehydrogenase (ACDH) of S. cerevisiae . This enzyme is thought to be involved in the production of acetate from which cytosolic acetyl‐CoA is then synthesized. Deletion of YPL061w was detrimental to the growth of haploid strains of yeast; an analysis of one deletion mutant revealed a maximum specific growth rate (in complex medium containing glucose) of one‐third of that displayed by the wild‐type strain. Mutants deleted in YPL061w were also unable to use ethanol as a carbon source. As expected, the cytosolic, Mg 2+ ‐activated ACDH activity had been lost from the mutants, although the mitochondrial, K + ‐activated ACDH was readily detected. YPL061w has been registered with the name of ALD6 in the Saccharomyces Genome Database and the nucleotide sequence submitted to GenBank as part of accession number U39205. © 1997 John Wiley & Sons, Ltd.