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Expression Profiles of Transcripts from 126 Open Reading Frames in the Entire Chromosome VI of Saccharomyces cerevisiae by Systematic Northern Analyses
Author(s) -
Naitou Masanori,
Hagiwara Hiroko,
Hanaoka Fumio,
Eki Toshihiko,
Murakami Yasufumi
Publication year - 1997
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199710)13:13<1275::aid-yea172>3.0.co;2-7
Subject(s) - orfs , biology , open reading frame , genetics , gene , saccharomyces cerevisiae , transcription (linguistics) , peptide sequence , linguistics , philosophy
Chromosome VI of Saccharomyces cerevisiae contains 126 open reading frames (ORFs), and the functions of proteins encoded by 80 ORFs are still unknown. In this report, we have systematically examined the expression profiles of all 126 ORFs on chromosome VI under five kinds of growth conditions by quantitative Northern hybridization. A series of Northern analyses and reverse transcription polymerase chain reactions have revealed that more than 64 novel ORFs are transcribed. Two ORFs (YFL059w and YFR011c) are specifically expressed in the presence of galactose. Two ORFs (YFL012w and YFR032c) are specifically transcribed in sporulation. Six ORFs (YFL049w, YFL035c, YFL010c, YFR006w, YFR010w and YFR017c) are abundantly expressed in many growth conditions. © 1997 John Wiley & Sons, Ltd.