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Isolation of Three Contiguous Genes, ACR1 , ACR2 and ACR3 , Involved in Resistance to Arsenic Compounds in the Yeast Saccharomyces cerevisiae
Author(s) -
Bobrowicz Piotr,
Wysocki Robert,
Owsianik Grzegorz,
Goffeau André,
Ułaszewski Stanisław
Publication year - 1997
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199707)13:9<819::aid-yea142>3.0.co;2-y
Subject(s) - biology , gene , plasmid , saccharomyces cerevisiae , operon , arsenite , genetics , gene product , arsenate , structural gene , microbiology and biotechnology , gene expression , arsenic , escherichia coli , materials science , metallurgy
Abstract A 4·2 kb region from Saccharomyces cerevisiae chromosome XVI was isolated as a yeast fragment conferring resistance to 7 m M ‐sodium arsenite (NaAsO 2 ), when put on a multicopy plasmid. Homology searches revealed a cluster of three new open reading frames named ACR1 , ACR2 and ACR3 . The hypothetical product of the ACR1 gene is similar to the transcriptional regulatory proteins, encoded by YAP1 , and YAP2 genes from S. cerevisiae . Disruption of the ACR1 gene conduces to an arsenite and arsenate hypersensitivity phenotype. The ACR2 gene is indispensable for arsenate but not for arsenite resistance. The hypothetical product of the ACR3 gene shows high similarity to the hypothetical membrane protein encoded by Bacillus subtilis ORF1 of the skin element and weak similarity to the ArsB membrane protein of the Staphylococcus aureus arsenical‐resistance operon. Overexpression of the ACR3 gene confers an arsenite‐ but not an arsenate‐resistance phenotype. The presence of ACR3 together with ACR2 on a multicopy plasmid expands the resistance phenotype into arsenate. These findings suggest that all three novel genes: ACR1 , ACR2 and ACR3 are involved in the arsenical‐resistance phenomenon in S. cerevisiae . © 1997 John Wiley & Sons, Ltd.