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Characterization of Two New Genes Down‐regulated by α‐factor
Author(s) -
Seidel Johannes,
Tanner Widmar
Publication year - 1997
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199707)13:9<809::aid-yea141>3.0.co;2-1
Subject(s) - biology , gene , open reading frame , signal peptide , complementary dna , peptide sequence , microbiology and biotechnology , gene product , amino acid , saccharomyces cerevisiae , genetics , gene expression
To detect genes directly down‐regulated by α‐factor, 55 000 plaque‐forming units of a Saccharomyces cerevisiae λgt10 gene bank were differentially screened with cDNA of cells treated with α‐factor for 20 min. Two new genes were detected in this way, called α0·5 and α0·6. The former is transiently down‐regulated by α‐factor; it is very highly transcribed in late exponential‐phase cells. The gene, located on the right arm of chromosome XIII, codes for a 59 amino‐acid protein with a signal peptide. The protein has been shown with an antibody to be present in the membrane fraction. The gene has also been cloned as HOR7 (hyperosmolarity‐responsive protein; Hirayama et al ., 1995). No other homologous sequences have been detected in the yeast genome. α0·6, located on the right arm of chromosome XII, corresponds to the open reading frame YLR110c; it codes for a 133 amino‐acid protein containing a signal peptide. Its derived amino‐acid sequence is homologous to the N‐terminal half of the SED1 gene product. SED1 , when overexpressed, is able to suppress a defect in the HDEL receptor coded for by the ERD2 gene (Hardwick and Pelham, 1994); however, α0·6 is not able to do so. The disruption of α0·5 or α0·6 does not lead to a special phenotype. © 1997 John Wiley & Sons, Ltd.