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DNA Sequencing and Analysis of 130 kb from Yeast Chromosome XV
Author(s) -
VOSS HARTMUT,
BENES VLADIMIR,
ANDRADE MIGUEL A.,
VALENCIA ALFONSO,
RECHMANN STEFANIE,
TEODORU CRISTINA,
SCHWAGER CHRISTIAN,
PACES VACLAV,
SANDER CHRIS,
ANSORGE WILHELM
Publication year - 1997
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19970615)13:7<655::aid-yea120>3.0.co;2-i
Subject(s) - orfs , biology , genetics , gene , nucleic acid sequence , homology (biology) , sequence analysis , open reading frame , elongation factor , saccharomyces cerevisiae , microbiology and biotechnology , peptide sequence , rna , ribosome
We have determined the nucleotide sequence of 129 524 bases of yeast ( Saccharomyces cerevisiae ) chromosome XV. Sequence analysis revealed the presence of 59 non‐overlapping open reading frames (ORFs) of length >300 bp, three tRNA genes, four delta elements and one Ty‐element. Among the 21 previously known yeast genes (36% of all ORFs in this fragment) were nucleoporin (NUP1), ras protein (RAS1), RNA polymerase III (RPC1) and elongation factor 2 (EF2). Further, 31 ORFs (53% of the total) were found to be homologous to known protein or DNA sequences, or sequence patterns. For seven ORFs (11% of the total) no homology was found. Among the most interesting protein identifications in this DNA fragment are an inositol polyphosphatase, the second gene of this type found in yeast (homologous to the human OCRL gene involved in Lowe's syndrome), a new ADP ribosylation factor of the arf6 subfamily, the first protein containing three C2 domains, and an ORF similar to a Bacillus subtilis cell‐cycle related protein. For each ORF detailed sequence analysis was carried out, with a full consideration of its biological function and pointing out key regions of interest for further functional analysis. The sequence has been submitted to the EMBL data library under Accession Number X94335.© 1997 John Wiley & Sons, Ltd.