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Serine Palmitoyltransferase ( scs 1/ lcb 2) Mutants have Elevated Copy Number of the L‐A dsRNA Virus
Author(s) -
GARNEPUDI VARSHA R.,
ZHAO CHUN,
BEELER TROY,
DUNN TERESA
Publication year - 1997
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19970330)13:4<299::aid-yea90>3.0.co;2-p
Subject(s) - biology , mutant , virology , genetics , microbiology and biotechnology , gene
Abstract The microsomal fraction isolated from serine palmitoyltransferase ( lcb2/scs1 ) mutants is enriched in a 90 kDa protein. The protein was identified as the major coat (Gag) protein of the L‐A dsRNA virus particles by partial sequencing and by its interaction with anti‐Gag antibodies. The total amount of Gag in whole‐cell lysates of scs1/lcb2 mutant cells is greater than in wild‐type lystes indicating that the enrichment of the protein in the microsomal fraction of scs1/lcb2 mutant cells may result from increased copy number of the L‐A dsRNA virus. This is supported by the finding that the mutants also have increased levels of L‐A dsRNA. Altered sphingolipid synthesis in the scs1 mutant cells appears to increase the copy number of the L‐A viral particles. © 1997 John Wiley & Sons, Ltd.