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An 18·3 kb DNA Fragment from Yeast Chromosome VII Carries Four Unknown Open Reading Frames, the Gene for an Asn Synthetase, Remnants of Ty and Three tRNA Genes
Author(s) -
VAN DYCK LUC,
TETTELIN HERVE,
PURNELLE BENEDICTE,
GOFFEAU ANDRE
Publication year - 1997
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199702)13:2<171::aid-yea57>3.0.co;2-v
Subject(s) - orfs , biology , open reading frame , genetics , schizosaccharomyces pombe , gene , homology (biology) , saccharomyces cerevisiae , peptide sequence
An 18·3 kb DNA segment from yeast Saccharomyces cerevisiae chromosome VII encompasses the previously characterized MEP1, NUP57 and PPT1 genes as well as seven new open reading frames (ORFs) of at least 100 residues. G6358 is an ubiquitous glutamine‐dependant asparagine synthase. G6362 is a membrane protein highly homologous to a protein of unknown function in the yeast Schizosaccharomyces pombe . Three ORFs (G6324, G6335 and G6365) have no significant homology with previously reported proteins or characteristic motifs. G6321 and G6359 , enclosed in longer ORFs, are not likely to be coding. The segment also contains tRNA genes for Asn, Arg and Ile as well as a sigma element and two solo deltas. ORFs and genetic elements are named according to a preliminary working nomenclature. The sequence is recorded in GenBank TM /EMBL under Accession Number X83099.©1997 John Wiley & Sons, Ltd.