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Histone H1 in Saccharomyces cerevisiae
Author(s) -
USHINSKY S. C.,
BUSSEY H.,
AHMED A. A.,
WANG Y.,
FRIESEN J.,
WILLIAMS B. A.,
STORMS R. K.
Publication year - 1997
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199702)13:2<151::aid-yea94>3.0.co;2-5
Subject(s) - biology , histone h1 , histone h2a , saccharomyces cerevisiae , sap30 , histone , microbiology and biotechnology , gene , yeast , genetics , hdac11
The existence of histone H1 in the yeast, Saccharomyces cerevisiae , has long been debated. In this report we describe the presence of histone H1 in yeast. YPL127c , a gene encoding a protein with a high degree of similarity to histone H1 from other species was sequenced as part of the contribution of the Montreal Yeast Genome Sequencing Group to chromosome XVI. To reflect this similarity, the gene designation has been changed to HHO1 (Histone H One). The HHO1 gene is highly expressed as poly A + RNA in yeast. Although deletion of this gene had no detectable effect on cell growth, viability or mating, it significantly altered the expression of β‐galactosidase from a CYC1 ‐lacZ reporter. Fluorescence observed in cells expressing a histone H1‐GFP protein fusion indicated that histone H1 is localized to the nucleus.©1997 John Wiley & Sons, Ltd.