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The Essential Schizosaccharomyces pombe gpi1 + Gene Complements a Bakers' Yeast GPI Anchoring Mutant and is Required for Efficient Cell Separation
Author(s) -
COLUSSI PAUL A.,
ORLEAN PETER
Publication year - 1997
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199702)13:2<139::aid-yea69>3.0.co;2-s
Subject(s) - schizosaccharomyces pombe , biology , saccharomyces cerevisiae , ura3 , complementation , cytokinesis , mutant , phosphatidylinositol , yeast , schizosaccharomyces , cell division , gene , microbiology and biotechnology , biochemistry , cell , signal transduction
The Schizosaccharomyces pombe gpi1 + gene was cloned by complementation of the Saccharomyces cerevisiae gpi1 mutant, which has temperature‐sensitive defects in growth and glycosyl phosphatidylinositol (GPI) membrane anchoring of protein, and which is defective in vitro in the first step in GPI anchor assembly, the formation of N ‐acetylglucosaminyl phosphatidylinositol (GlcNAc‐PI). S. pombe gpi1 + encodes a protein with 29% identity to amino acids 87–609 of the S. cerevisiae protein, and is the functional homolog of the S. cerevisiae Gpi1 protein, for it restores [ 3 H]inositol‐labelling of protein and in vitro GlcNAc‐PI synthetic activity to both S. cerevisiae gpi1 and gpi1::URA3 cells. Disruption of gpi1 + is lethal. Haploid Δ gpi1 + ::his7 + spores germinate, but proceed through no more than three rounds of cell division, many cells ceasing growth as binucleate, septate cells with thickened septa. These results indicate that GPI synthesis is an essential function in fission yeast, and suggest that GPI anchoring is also required for completion of cytokinesis. The nucleotide sequence reported will appear in the GenBank Nucleotide Sequence database under the Accession Number U77355.©1997 John Wiley & Sons, Ltd.