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The Sequence of a 36·7 kb Segment on the Left Arm of Chromosome IV from Saccharomyces cerevisiae Reveals 20 Non‐overlapping Open Reading Frames (ORFs) Including SIT4 , FAD1 , NAM1 , RNA11 , SIR2 , NAT1 , PRP9 , ACT2 and MPS1 and 11 New ORFs
Author(s) -
SARÉN ARIMATTI,
LAAMANEN PÄIVI,
LEJARCEGUI JAVIER BEASCOECHEA,
PAULIN LARS
Publication year - 1997
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199701)13:1<65::aid-yea50>3.0.co;2-t
Subject(s) - orfs , biology , open reading frame , genetics , homology (biology) , gene , nucleic acid sequence , saccharomyces cerevisiae , peptide sequence
A 36 688 bp fragment from the left arm of chromosome IV of Saccharomyces cerevisiae was sequenced. Sequence analysis identified 20 complete non‐overlapping open reading frames (ORFs) of at least 100 amino acids. Nine of these correspond to previously identified and sequenced genes: SIT4/PPH1, FAD1, NAM1/MTF2, RNA11, SIR2/MAR1, NAT1/AAA1, PRP9, ACT2 and MPS1/RPK1 . Three ORFs show homology to previously sequenced genes. One ORF exhibits a hypothetical yabO/yceC/yfiI family signature and one has the ATP‐dependent helicase signature of the DEAD and DEAH box families. Six ORFs show no appreciable homology to any proteins in the database. One of these is identical to yeast expressed sequence tags and therefore corresponds to an expressed gene. In addition, two partial ORFs and 11 ORFs that are totally internal and are not likely to be functional were detected. The sequence has been submitted to the EMBL data library under Accession Number Z71781. © 1997 by John Wiley & Sons, Ltd.