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Galactose‐inducible Expression Systems in Candida maltosa using Promoters of Newly‐isolated GAL1 and GAL10 Genes
Author(s) -
PARK SUN MEE,
OHKUMA MORIYA,
MASUDA YUTAKA,
OHTA AKINORI,
TAKAGI MASAMICHI
Publication year - 1997
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199701)13:1<21::aid-yea58>3.0.co;2-l
Subject(s) - biology , kluyveromyces lactis , gene , promoter , galactose , gene cluster , plasmid , microbiology and biotechnology , gene expression , galactokinase , reporter gene , expression vector , shuttle vector , genetics , recombinant dna , biochemistry , saccharomyces cerevisiae , vector (molecular biology) , escherichia coli
The GAL1 and GAL10 gene cluster encoding the enzymes of galactose utilization was isolated from an asporogenic yeast, Candida maltosa . The structure of the gene cluster in which both genes were divergently transcribed from the central promoter region resembled those of some other yeasts. The expression of both genes was strongly induced by galactose and repressed by glucose in the medium. Galactose‐inducible expression vectors in C. maltosa were constructed on low‐ and high‐copy number plasmids using the promoter regions of both genes. With these vectors and the β‐galactosidase gene from Kluyveromyces lactis as a reporter, galactose‐inducible expression was confirmed. Homologous overexpression of members of the cytochrome P‐450 gene family in C. maltosa was also successful by using a high‐copy‐number vector under the control of these promoters. © 1997 by John Wiley & Sons, Ltd.