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Analysis of a 22 956 bp region on the right arm of Saccharomyces cerevisiae chromosome XV
Author(s) -
Madania Ammar,
Poch Olivier,
Tarassov Ivan,
Winsor Barbara,
Martin Robert
Publication year - 1996
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199612)12:15<1563::aid-yea44>3.0.co;2-m
Subject(s) - orfs , biology , cosmid , genetics , open reading frame , gene , homology (biology) , nucleic acid sequence , chromosome , peptide sequence
We present here the sequence analysis of a DNA fragment (cosmid pUOA1258) located on the right arm of chromosome XV. The 22 956 bp sequence reveals 14 open reading frames (ORFs) longer than 300 bp and the 201 bp RPS33 gene. Among the 14 large ORFs, two overlapping frames are likely to be non‐expressed and one corresponds to the known GLN4 gene encoding glutaminyl‐tRNA synthetase. Two ORFs, O3571 and O3620, encode putative transcriptional regulators with a Zn(2)‐Cys(6) DNA binding domain characteristic of members of the GAL4 family. Among the nine remaining ORFs, five (O3568, O3575, O3590, O3615 and O3625) present significant similarity to proteins of unknown function and four (O3580, O3595, O3630 and O3635) lack homology to sequences present in the databases screened. This sequence has been deposited in the GenBank database under Accession Number U55021.