Premium
Nucleotide sequence analysis of a 40 kb segment on the right arm of yeast chromosome XV reveals 18 open reading frames including a new pyruvate kinase and three homologues to chromosome I genes
Author(s) -
Purnelle Bénédicte,
Goffeau André
Publication year - 1996
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199611)12:14<1475::aid-yea32>3.0.co;2-v
Subject(s) - biology , open reading frame , genetics , chromosome , nucleic acid sequence , reading (process) , sequence (biology) , microbiology and biotechnology , gene , peptide sequence , linguistics , philosophy
We have determined the nucleotide sequence of a 40 kb fragment from the right arm of chromosome XV of Saccharomyces cerevisiae . Subsequent analysis revealed 18 non‐overlapping open reading frames (ORFs) numbered from 06257 to 06357 , an ARS, two tRNA genes and a Ty2 with its flanking elements. Ten ORFs have been sequenced previously: TEA1, RPA43, RPA190, SGC1 (also called TYE7 ) REV1, PUT4, CIN1, MNE and MRE4 (also called MEK1 ). Among the others, two seem to code for a new pyruvate kinase and for a new ubiquitin‐conjugating enzyme; three have interesting homology with genes located on the left arm of chromosome I. This similarity with chromosome I extends to the left of the sequence presented here (Parle et al ., submitted to Yeast ). The homologous genes on the two chromosomes are placed in the same relative order. The complete nucleotide sequence of 40 295 bp and the deduced ORFs were submitted to the EMBL database (Accession Number X95720).