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Identification of a group‐i intron within the 25S rDNA from the yeast Arxula adeninivorans
Author(s) -
Rösel Harald,
Kunze Gotthard
Publication year - 1996
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19960930)12:12<1201::aid-yea1>3.0.co;2-t
Subject(s) - biology , intron , group i catalytic intron , schizosaccharomyces pombe , genetics , genbank , ribosomal dna , ribosomal rna , rna splicing , genomic dna , microbiology and biotechnology , nucleic acid sequence , dna , saccharomyces cerevisiae , gene , phylogenetic tree , rna
The 25S rDNA of the yeast Arxula adeninivorans LS3 has been cloned from a genomic library and sequenced. This DNA could be localized on chromosome 1 from A. adeninivorans and comprised 3790bp. The DNA sequence from this rDNA of the strain LS3 is very similar to the 25S rDNA of Candida albicans (91·7%), Saccharomyces cerevisiae (90·5%), Schizosaccharomyces pombe (83·8%) and Mucor racemosus (79·2%). Additionally a 411bp insertion could be localized within the 25S rDNA. This intervening sequence, which is devoid of any long open reading frame, is a group‐IC intron as revealed from its site of insertion, predicted secondary structure, and its self‐splicing capability. The Arxula intron is intermediate in structure and sequence between the ribosomal introns of Tetrahymena thermophila and C. albicans . The nucleotide sequence reported in this paper has been entered in the GenBank/EMBL data libraries and assigned Accession Number Z50840.