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The sequence of a 20·3 kb DNA fragment from the left arm of Saccharomyces cerevisiae chromosome IV contains the KIN28, MSS2, PHO2, POL3 and DUN1 genes, and six new open reading frames
Author(s) -
Saiz Julia E.,
Buitrago Maria J.,
Garcia Raul,
Revuelta Jose L.,
Del Rey Francisco
Publication year - 1996
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199609)12:10b<1077::aid-yea8>3.0.co;2-z
Subject(s) - biology , genetics , open reading frame , orfs , gene , homology (biology) , leucine zipper , complete sequence , dna , microbiology and biotechnology , nucleic acid sequence , peptide sequence , genome
We report the sequence of a 20 300 bp DNA fragment from the left arm of Saccharomyces cerevisiae chromosome IV. This segment contains 13 complete open reading frames (ORFs) and part of another ORF, altogether covering 84·2% of the entire sequence, five of which correspond to the previously characterized KIN28, MSS2, PHO2, POL3 / CDC2 and DUN1 genes. One putative protein, D2358p, shares considerable homology with an O‐sialoglycoprotein endopeptidase from Pasteurella haemolytica serotype A1. The putative product of D2325 contains the characteristic consensus motif of triacylglycerol lipases. D2320p and D2352p have a putative ‘leucine‐zipper’ structure and a RNA‐binding region Rnp‐1 signature, respectively. The sequence data have been submitted to EMBL data library under Accession Number X95644.