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Identification of a putative methylenetetrahydrofolate reductase by sequence analysis of a 6·8 kb DNA fragment of yeast chromosome VII
Author(s) -
Tizon Belen,
RodríguezTorres Ana M.,
RodríguezBelmonte Esther,
Cadahia Jose L.,
Cerdan Esperanza
Publication year - 1996
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199609)12:10b<1047::aid-yea991>3.0.co;2-n
Subject(s) - orfs , biology , genetics , genbank , accession number (library science) , nucleic acid sequence , open reading frame , gene , sequence analysis , microbiology and biotechnology , peptide sequence
We report the sequence analysis of a 6·8 kb DNA fragment from Saccharomyces cerevisiae chromosome VII. This sequence contains five open reading frames (ORFs) greater than 100 amino acids. There is also an incomplete ORF flanking one of the extremes, G2868, which is the 3′ end of the SCS3 gene (Hosaka et al ., 1994). The translated sequence of ORF G2882 shows similarity to the human methylenetetrahydrofolate reductase (Goyette et al ., 1994). ORF G2889 shows no significant homologies with the sequences compiled in databases. ORF G2893 corresponds to the gene SUP44 , coding for the yeast ribosomal protein S4 (All‐Robin et al ., 1990). G2873 and G2896 are internal ORFs. The whole sequence of the fragment is available at the EMBL nucleotide sequence database, GenBank and Data Bank of Japan under the Accession Number X94106.