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DNA sequence analysis of a 10 624 bp fragment of the left arm of chromosome XV from Saccharomyces cerevisiae reveals a RNA binding protein, a mitochondrial protein, two ribosomal proteins and two new open reading frames
Author(s) -
Lafuente María J.,
Gamo FranciscoJavier,
Gancedo Carlos
Publication year - 1996
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199609)12:10b<1041::aid-yea989>3.0.co;2-i
Subject(s) - biology , orfs , genetics , ribosomal protein , nucleic acid sequence , homology (biology) , ribosome , open reading frame , microbiology and biotechnology , gene , accession number (library science) , sequence analysis , peptide sequence , rna , genbank
We have determined the sequence of a 10 624 bp DNA segment located in the left arm of chromosome XV of Saccharomyces cerevisiae . The sequence contains eight open reading frames (ORFs) longer than 100 amino acids. Two of them do not present significant homology with sequences found in the databases. The product of ORF o0553 is identical to the protein encoded by the gene SMF1 . Internal to it there is another ORF, o0555 that is apparently expressed. The proteins encoded by ORFs o0559 and o0565 are identical to ribosomal proteins S19.e and L18 respectively. ORF o0550 encodes a protein with an RNA binding signature including RNP motifs and stretches rich in asparagine, glutamine and arginine. The nucleotide sequence determined has been deposited in the EMBL data library under Accession Number X95258.