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Sequence analysis of a 13·4 kbp fragment from the left arm of chromosome XV reveals a malate dehydrogenase gene, a putative Ser/Thr protein kinase, the ribosomal L25 gene and four new open reading frames
Author(s) -
Casamayor Antonio,
Khalid Hajji,
Balcells Lluís,
Aldea Martí,
Casas Celia,
Herrero Enrique,
Ariño Joaquín
Publication year - 1996
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199609)12:10b<1013::aid-yea980>3.0.co;2-5
Subject(s) - biology , genetics , gene , ribosomal protein , open reading frame , microbiology and biotechnology , nucleic acid sequence , accession number (library science) , orfs , start codon , peptide sequence , genbank , ribosome , nucleotide , rna
A 13 421 bp fragment located near the left telomere of chromosome XV (cosmid pEOA461) has been sequenced. Seven non‐overlapping open reading frames (ORFs) encoding polypeptides longer than 100 residues have been found (AOB859, AOC184, AOE375, AOX142i, AOE423, AOA476 and AOE433). An additional ORF (AOE131) is found within AOA476. Three of them (AOC184, AOA476 and AOE433) show no remarkable identity with proteins deposited in the data banks. ORF AOB859 is quite similar to a hypothetical yeast protein of similar size located in chromosome VI, particularly within the C‐terminal half. AOE375 encodes a new member of the glycogen synthase kinase‐3 subfamily of Ser/Thr protein kinases. AOX142i is the gene encoding the previously described ribosomal protein L25. AOE423 codes for a protein virtually identical to the MDH2 malate dehydrogenase isozyme. However, our DNA sequence shows a single one‐base insertion upstream of the reported initiating codon. This would produce a larger ORF by extending 46 residues the N‐terminus of the protein. The existence of this insertion has been confirmed in three different yeast strains, including FY1679. The complete nucleotide sequence of the 13·4 kbp fragment has been deposited at the DNA databases (Accession Number U41293).