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Sequence and analysis of a 33 kb fragment from the right arm of chromosome XV of the yeast Saccharomyces cerevisiae
Author(s) -
Galisson Frédérique,
Dujon Bernard
Publication year - 1996
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199607)12:9<877::aid-yea969>3.0.co;2-s
Subject(s) - orfs , biology , genetics , nucleic acid sequence , saccharomyces cerevisiae , homology (biology) , open reading frame , cosmid , peptide sequence , gene
We have determined the nucleotide sequence of a cosmid (pEOA423) from chromosome XV of Saccharomyces cerevisiae . Analysis of the 33,173 bp sequence reveals the presence of 20 putative open reading frames (ORFs). Five of them correspond to previously known genes ( MGM1, STE4, CDC44, STE13, RPB8 ). The previously published nucleotide sequences are in perfect agreement with our sequence except for STE4 and MGM1 . In the latter case, 59 amino acids were truncated from the published protein at its N‐terminal end due to a frameshift. The putative translation products of six other ORFs exhibit significant homology with protein sequences in public databases: O50 03 and O50 17 products are homologs of the ANC1 and MIP1 proteins of S. cerevisiae , respectively; O50 05 product is similar to that of a protein of unknown function from Myxococcus xanthus ; O50 12 product is probably a new ATP/ADP carrier; O50 13 product shows homology with group II tRNA synthetases; and the O50 16 product exhibits strong similarity with the N‐terminal domain of the NifU proteins from several prokaryotes. The remaining nine ORFs show no significant similarity. Among these, two contiguous ORFs (O50 19 and O50 20) are very similar to each other, suggesting an ancient tandem duplication. The 33,173 bp sequence has been submitted to EMBL (Accession Number: X92441).