Premium
Sustained oscillations in free‐energy state and hexose phosphates in yeast
Author(s) -
Richard Peter,
Teusink Bas,
Hemker Mirte B.,
van Dam Karel,
Westerhoff Hans V.
Publication year - 1996
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19960630)12:8<731::aid-yea961>3.0.co;2-z
Subject(s) - glycolysis , biology , energy charge , hexose , biochemistry , fructose , adenylate kinase , yeast , saccharomyces cerevisiae , population , adenine nucleotide , intracellular , metabolism , nucleotide , enzyme , gene , demography , sociology
In a population of intact cells of the yeast Saccharomyces cerevisiae the dynamics of glycolytic metabolism were investigated under the condition of sustained oscillations. At 5‐s intervals cells were quenched in −40°C methanol, extracted and the intracellular concentrations of glycolytic metabolites, adenine nucleotides and phosphate were analysed. Oscillations were found for the glycolytic intermediates glucose 6‐phosphate, fructose 6‐phosphate and fructose 1,6‐bisphosphate. At variance with earlier reports on transient glycolytic oscillations, some intermediates further down the glycolytic pathway did not oscillate significantly, even though NADH did. In addition, the adenylate energy charge and the free energy of ATP hydrolysis oscillated significantly. Dynamic coupling through the latter may be responsible for this effective compartmentation of glycolytic dynamics.