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HST1 , a new member of the SIR2 family of genes
Author(s) -
Derbyshire Myra K.,
Weinstock Keith G.,
Strathern Jeffrey N.
Publication year - 1996
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19960615)12:7<631::aid-yea960>3.0.co;2-8
Subject(s) - genbank , biology , accession number (library science) , gene , genetics , saccharomyces cerevisiae , sequence (biology) , genome , gene silencing , genomic dna , computational biology
Abstract A novel Saccharomyces cerevisiae gene, HST1 , was identified from among anonymous cDNAs and the complete corresponding genomic clone was isolated and sequenced. HST1 is very closely related to SIR2 , showing 71% sequence identity over 84% of its length. Polymerase chain reaction with degenerate primers on S. cerevisiae DNA identified three additional SIR2 ‐related genes designated HST2, HST3 and HST4 . The sequences of HST2, HST3 and HST4 correspond to sequences previously released by the S. cerevisiae genome sequencing project as U33335, NCBI gi:965078; X87331, NCBI gi:829135; and Z48784, YD9346.03, respectively. Disruption of HST1 has shown no phenotype with respect to mechanisms in which SIR2 has a role, namely, regional silencing of HML α, or in rDNA recombination. The sequence of HST1 has been deposited in the DDBJ, EMBL and GenBank at NCBI database under Accession Number L47120.