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FEN2 : A gene implicated in the catabolite repression‐mediated regulation of ergosterol biosynthesis in yeast
Author(s) -
Marcireau Christophe,
Joets Johann,
Pousset Dominique,
Guilloton Michel,
Karst Francis
Publication year - 1996
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199605)12:6<531::aid-yea934>3.0.co;2-e
Subject(s) - catabolite repression , biology , mutant , ergosterol , saccharomyces cerevisiae , yeast , biochemistry , open reading frame , overproduction , biosynthesis , gene , psychological repression , gene product , genetics , gene expression , peptide sequence
We have isolated and characterized a pleiotropic recessive mutation, fen2 ‐ 1 , that causes resistance to fenpropimorph and a low level of ergosterol in Saccharomyces cerevisiae . Ergosterol synthesis in the mutant strain was 5·5‐fold slower than in the wild type; however, in vitro assays of the enzymes involved in ergosterol biosynthesis could not account for this low rate in the mutant. The mutant phenotype was expressed only in media exerting both carbon and nitrogen catabolite repression. To our knowledge, this is the first locus in yeast that reveals a concerted regulation between different pathways (carbon and nitrogen catabolite repression and/or general control of amino acid biosynthesis and ergosterol biosynthesis). The yeast gene FEN2 has been isolated and contains an open reading frame (ORF) of 512 codons. This ORF was found to be identical to YCR28C of chromosome III. A possible function of the FEN2 gene product in yeast is discussed.