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Selection of yeast cells with a higher plasmid copy number in a Saccharomyces cerevisiae autoselection system
Author(s) -
Compagno Concetta,
Porro Danilo,
Radice Stefania,
Martegani Enzo,
Ranzi Bianca Maria
Publication year - 1996
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19960315)12:3<199::aid-yea895>3.0.co;2-u
Subject(s) - biology , plasmid , saccharomyces cerevisiae , yeast , population , heterologous , genetics , selection (genetic algorithm) , experimental evolution , gene , microbiology and biotechnology , demography , artificial intelligence , sociology , computer science
Autoselection systems allow the selection of a genetically engineered population independently of the growth medium composition. The structure of a Saccharomyces cerevisiae population transformed with an autoselection plasmid, in which a carbon‐source‐dependent modulation of the plasmid copy number occurs, was analysed. By means of flow cytometric procedures we tested the cell viability, dynamics of growth and heterologous protein production at single cell level. Such analyses allow the identification and the tracking of a specific cellular sub‐population with a higher plasmid copy number which arises after the carbon source shift. The effects of the cellular plasmid distribution on the dynamics of growth are also discussed.