Premium
Fifteen open reading frames in a 30·8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae
Author(s) -
Eki Toshihiko,
Naitou Masanori,
Hagiwara Hiroko,
Abe Makoto,
Ozawa Masashi,
Sasanuma ShunIchi,
Sasanuma Motoe,
Tsuchiya Yukari,
Shibata Takehiko,
Watanabe Koji,
Ono Ayako,
Yamazaki MasaAki,
Tashiro Hiroyuki,
Hanaoka Fumio,
Murakami Yasufumi
Publication year - 1996
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199602)12:2<177::aid-yea896>3.0.co;2-a
Subject(s) - biology , open reading frame , long arm , genetics , reading (process) , chromosome , linguistics , gene , peptide sequence , philosophy
The nucleotide sequence of cosmid clone 9765, which contains 30·8 kb of the right arm of chromosome VI, was determined. Both strands were sequenced, with an average redundancy of 8·17 per base pair by both dye primer and dye terminator cycle sequencing methods. The G+C content of the sequence was found to be 40·3%. Fifteen open reading frames (ORFs) greater than 100 amino acids and one tRNA‐Tyr gene ( SUP6 ) were detected. Seven of the ORFs were found to encode previously identified genes ( HIS2, CDC14, MET10, SMC2, QCR6, PH04 and CDC26 ). One ORF, 9765orfF010, was found to encode a new member of the Snf2/Rad54 helicase family. Three ORFs (9765orfR002, 9765orfR011 and 9765orfR013) were found to be homologous with Schizosaccharomyces pombe polyadenylate binding protein, Escherichia coli hypothetical 38·1‐kDa protein in the BCR 5′ region, and transcription regulatory protein Swi3, respectively. The sequence may be found in the DDBJ, EMBL and GenBank nucleotide sequence databases under Accession Number D44602.