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pMPY‐ZAP: A reusable polymerase chain reaction‐directed gene disruption cassette for Saccharomyces cerevisiae
Author(s) -
Schneider B. L.,
Steiner B.,
Seufert W.,
Futcher A. B.
Publication year - 1996
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199602)12:2<129::aid-yea891>3.0.co;2-o
Subject(s) - ura3 , biology , saccharomyces cerevisiae , gene , polymerase chain reaction , genetics , cloning (programming) , gene cassette , computational biology , plasmid , computer science , programming language , integron
Gene disruption is an important method for genetic analysis in Saccharomyces cerevisiae . We have designed a polymerase chain reaction‐directed gene disruption cassette that allows rapid disruption of genes in S. cerevisiae without previously cloning them. In addition, this cassette allows recycling of URA3 , generating gene disruptions without the permanent loss of the ura3 marker. An indefinite number of disruptions can therefore be made in the same strain.