Premium
Nucleotide sequence analysis of a 32,500 bp region of the right arm of Saccharomyces cerevisiae chromosome IV
Author(s) -
Brandt Petra,
Ramlow Silke,
Otto Beate,
Bloecker Helmut
Publication year - 1996
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199601)12:1<85::aid-yea890>3.0.co;2-u
Subject(s) - orfs , biology , cosmid , genetics , subcloning , gene , saccharomyces cerevisiae , open reading frame , transfer rna , nucleic acid sequence , accession number (library science) , genbank , peptide sequence , plasmid , rna
We have sequenced a region containing 32.5 kb of the right arm of chromosome IV of Saccharomyces cerevisiae . Twenty open reading frames (ORFs) greater than 100 amino acids could be identified in this region. Six ORFs correspond to known yeast genes, including DOA4, UBC5 and UBC3 , the gene products of which are involved in ubiquitin metabolism. UBC5 is preceded by the two tRNA genes tRNA‐ Arg2 and tRNA‐ Asp . Six genes were discovered with homologies to non‐yeast genes or with homologies to other yeast ORFs. One of these could be identified as ribosomal protein gene RPS13 . The putative function of eight ORFs remains unclear because comparison to different DNA or protein databases revealed no significant patterns. The sequence from cosmid 2F21 was obtained entirely by a combined subcloning and walking primer strategy, and has been deposited in the EMBL data library under Accession Number X84162.